Literature summary extracted from

Richards, N.G.; Kilberg, M.S.
Asparagine synthetase chemotherapy (2006), Annu. Rev. Biochem., 75, 629-654.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
6.3.1.1	Phytohemagglutinin	-	Escherichia coli
6.3.5.4	Phytohemagglutinin	-	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
6.3.5.4	E348D	mutant forms two molecules of diphosphate per asparagine	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
6.3.1.1	sulfoximine	poor inhibitor	Escherichia coli
6.3.5.4	beta-asparaginyladenylate	-	Homo sapiens
6.3.5.4	mupirocin	-	Homo sapiens
6.3.5.4	phosmidosine	-	Homo sapiens
6.3.5.4	sulfoximine adenylate	most potent inhibitor	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.3.1.1	Escherichia coli	-	-	-
6.3.5.4	Escherichia coli	-	-	-
6.3.5.4	Homo sapiens	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
6.3.5.4	leukemia cell	-	Homo sapiens	-
6.3.5.4	MOLT-4 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.3.1.1	ATP + L-Asp + NH4+	-	Escherichia coli	AMP + diphosphate + Asn	-	?
6.3.5.4	ATP + L-Asp + L-Gln	-	Escherichia coli	AMP + diphosphate + L-Asn + L-Glu	-	?
6.3.5.4	ATP + L-aspartate + L-glutamine + H2O	-	Homo sapiens	AMP + diphosphate + L-asparagine + L-glutamate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.3.1.1	ammonia-dependent ASNS	-	Escherichia coli
6.3.1.1	AS-A	-	Escherichia coli
6.3.1.1	asparagine synthetase, ammonia-dependent	-	Escherichia coli
6.3.5.4	AS-B	-	Escherichia coli
6.3.5.4	ASNS	-	Homo sapiens
6.3.5.4	Asparagine synthetase	-	Escherichia coli
6.3.5.4	asparagine synthetase, glutamine-dependent	-	Homo sapiens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
6.3.5.4	ATP	-	Escherichia coli

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
6.3.5.4	0.000285	-	sulfoximine adenylate	-	Homo sapiens

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Release 2023.1 (January 2023)